Deoxynivalenol(DON) ELISA test kit
Catalog No. LSY-10031
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Deoxynivalenol in sample. The coupling antigen is pre-coated on the micro-well stripes. The Deoxynivalenol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Deoxynivalenol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Deoxynivalenol in the sample. This value is compared to the standard curve and the Deoxynivalenol residue is subsequently obtained.

2. Technical specifications
Sensitivity: 5 ppb (µg/kg)
Incubator temperature: 37℃
Incubator time: 30min
Detection limit:
Wheat, feed……………………………………………………………………...100ppb
Cross-reaction rate:
Deoxynivalenol… ……..100%
Zearalanol ………………………………………………………………… < 1%
Recovery rate:
Wheat, feed …….……………………………………………….………………70%-120%

3. Components

Code Contents 96T Packing
1 Micro-well strips 12×8 well
2 6× standard solution 6×1mL
3 Enzyme conjugate 7 mL
4 Antibody working solution 7 mL
5 Substrate A solution 7 mL
6 Substrate B solution 7 mL
7 Stop solution 7 mL
8 20× concentrated washing buffer 40 mL
9 2× DON diluent 40mL
Note: A、B、C、D、E、F the 6 standards solution: 0 ppb, 5 ppb, 15 ppb,45ppb, 135ppb, 405 ppb, use directly.